Prevention of mutagenic
oxidative DNA damage
by Brussels Sprouts.
The alleged cancer preventive effects of cruciferous vegetables could be related to protection from
mutagenic oxidative DNA damage.
We have studied the effects of Brussels sprouts, some non-cruciferous vegetables and
isolated glucosinolates on spontaneous and induced oxidative DNA damage in terms of
8-oxo7,8-dihydro-2’-deoxyguanosine (8-oxodG) in groups of 6-8 male Wistar rats.
Excess oxidative DNA damage was induced by 2-nitropropane (2-NP 100 mg/kg).
Four days oral
administration of 3g of cooked brussels sprouts homogenate reduced the spontaneous urinary
8-oxodG excretion by 31% (p<0.05) where as raw sprouts, beans and endive (1:1), isolated indolyl glucosinolates and breakdown products had no significant effect.
An aqueous
extract of cooked brussels sprouts (corresponding to 6.7 g vegetable per day for 4 days)
decreased the spontaneous 8-xoxdG excretion from 92+/-12 to 52+/-15 pmol/24 h (p<0.05).
After 2-NP administration the 8-oxodG excretion was increased to 132+/-26 pmol/24 h
(p<0.05) whereas pretreatment with the sprouts extracts reduced this to 102+/-30
pmol/24 h (p<0.05). The spontaneous level of 8-oxodG in nuclear DNA from liver and bone
marrow was not significantly affected by the sprouts extract whereas the level decreased
by 27% in the kidney (p<0.05).
In the liver 2-NP increased the 8-oxodG levels in nuclear DNA 8.7 and 3.8 times (p<0.05) 6 and 24 h after dose, respectively. The sprouts extract reduced this increase by 57% (p<0.05) at 6 h whereas there was no significant effect at 24 h.
In the kidneys 2-NP increased the 8-oxodG levels 2.2 and 1.2 times (p<0.05) and 6 and 24 h after dose, respectively.
Pretreatment with the sprouts extract abolished these increases (p<0.05).
Similarly, in the bone marrow the extract protected completely (p<0.05) against a 4.9-fold 2-NP induced increase (p<0.05) in the 8-oxodG level.
These findings demonstrate that cooked brussels sprouts contain bioactive substance(s) with a potential for reducing the physiological as well as oxidative stress induced oxidative DNA damage in rats.
This could explain the suggested cancer preventive effect of cruciferous vegetables.
The correspondence between the urinary excretion and 9-oxodG levels in 2-NP target organs supports its being the main repair product that reflects the rate of guanine oxidation in DNA.
Deng, X.S.; Tuo, J.; Poulsen, H.E. Loft, S. Department of Pharmacology, University of Copenhagen, Panum, Denmark. Free Radic Res 1998 Mar; 28(3):323-33
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